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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was discovered to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a critical role in advertising and marketing mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, happen to be explained. Two added splice variants were reported but specific nucleotide sequence facts will not be nevertheless offered.
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Blocking peptides are peptides that bind particularly to your concentrate on antibody and block antibody binding. These peptide ordinarily is made up of the epitope regarded through the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to ensure the absolute best final results are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining from the blocked antibody versus the antibody by itself, you can see which staining is distinct.
Goal Details Cyclin E belongs towards the highly conserved cyclin family, whose users are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards more info the temporal coordination of each and every mitotic celebration. Cyclin E types a fancy with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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